Using DNA hybridization with probes corresponding to different regions of the Y chromosome, Page discovered that sex reversed males carried genes from a 140 kilobase region on the short arm of the Y chromosome (Figure 1). Presumably, this region had been transferred to the X chromosome during a translocation (Page et al., 1985). Subsequent experiments narrowed down this region (McLaren, 1991) and found that one gene, the sex determining region of the Y, or SRY, was the master regulator of sex determination.
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